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目的 探讨急性应激对大鼠脑内5-羟色胺1A(5-HT1A)受体mRNA表达的影响。方法 将12只雄性Sprague-Dawley大鼠随机分为应激组和对照组,每组6只。根据5-HT1A受体互补DNA(eDNA)序列合成相应的特异性引物,用聚合酶链反应(PCR)法观察强迫游泳应激后3 h大鼠海马、下丘脑和中脑5-HT1A受体mRNA表达,并测定各脑区阳性电泳条带密度与β肌动蛋白(β-actin)密度的百分比。结果 应激后3 h,大鼠下丘脑5-HT1A受体mRNA表达相对水平为(64.3±6.7)％,显著低于对照组(78.9±8.7)％(t=3.263.P<0.05);海马、中脑5-HT1A受体mRNA表达相对水平分别为41.5％±7.1％、37.4％±5.6％,均分别显著低于对照组64.8％±9.6％、63.9％±6.3％(t分别为4.782、7.701,P均<0.01)。结论 急性应激后大鼠海马、下丘脑和中脑5-HT1A受体mRNA的表达降低。
Objective To investigate the effect of acute stress on the expression of 5-HT1A receptor mRNA in rat brain. Methods Twelve male Sprague-Dawley rats were randomly divided into stress group and control group, with 6 rats in each group. The corresponding specific primers were synthesized according to the sequence of 5-HT1A receptor complementary DNA (eDNA), and the 5-HT1A receptor in the hippocampus, hypothalamus and midbrain was observed 3 h after forced swimming stress by polymerase chain reaction (PCR) mRNA expression, and determine the brain area positive electrophoresis strip density and β-actin (β-actin) density percentage. Results The mRNA level of 5-HT1A receptor in the hypothalamus of rats was (64.3 ± 6.7)% at 3 h after stress, which was significantly lower than that in the control group (78.9 ± 8.7)% (t = 3.263.P <0.05) , While the relative levels of 5-HT1A receptor mRNA in midbrain were 41.5% ± 7.1% and 37.4% ± 5.6% respectively, which were significantly lower than 64.8% ± 9.6% and 63.9% ± 6.3% in control group (t = 4.782, 7.701, P <0.01). Conclusions After acute stress, the expression of 5-HT1A receptor mRNA in hippocampus, hypothalamus and midbrain decreased.