Hairpin相关论文
Reversing multidrug resistance by RNA interference through the suppression of MDR1 gene in human hep
AIM: To reverse the multidrug resistance (MDR) by RNA interference (RNAi)-mediated MDR1 suppression in hep-atoma cells. ......
This research presented an annealing strategy to identify hairpin DNA from coil DNA with the same base composition but d......
Sensitive detection of DNA methyltransferase activity based on rolling circle amplification technolo
This work develops a fluorescence approach for sensitive detection of DNA methyltransferase activity based on endonuclea......
A highly sensitive chemiluminescence sensor for detecting mercury(Ⅱ) ions: a combination of Exonucle
In this paper, we report a highly sensitive chemiluminescence(CL) sensor for Hg2+ ions based on thymine-Hg2+-thymine(T-H......
Disposable paper-based bipolar electrode array for multiplexed electrochemiluminescence detection of
A novel disposable paper-based bipolar electrode(BPE) array is fabricated for multiplexed electrochemiluminescence(ECL) ......
Polymers exhibit extended structures at high temperatures or in good solvents and collapsed configurations at low temper......
Modeling the temperature-dependent peptide vibrational spectra based on implicit-solvent model and e
We herein review our studies on simulating the thermal unfolding Fourier transform infrared and two-dimensional infrared......
Tumor necrosis factor-alpha(TNF-α) is a type of critical pro-inflammatory cytokines,which participates in numerous cell......
MAYBE I should be grateful to my mother. When I was very young, she taught me how to knit socks. Using a kind of white ......
One simple and versatile method is established for the synthesis of DNA recognition molecules-polyamides containing alte......
AIM:To study the effect of siRNA expressed from DNA vectoron HBV replication.METHODS:Human U6 promoter was amplified fr......
RNA interference by expression of short hairpin RNAs suppresses bcl-xL gene expression in nasopharyn
Aim:To evaluate a new plasmid mediated RNA interference(RNAi)system andinvestigate whether knock-down of bcl-xL by shor......
Selective DNA detection at Zeptomole level based on coulometric measurement of gold nanoparticle-med
A selective DNA sensing with zeptomole detection level is developed based on coulometric measurement of gold nanoparticl......
AT1a Receptor Has Interacted with Angiotensin-converting Enzymes 2 mRNA Expression in Mouse Brainste
Objectives To examine in vivo interactions between angiotensin Ⅱ (Ang Ⅱ) AT1a receptor (AT1aR), angiotensin-converting......
This study was designed to explore the RNA interference technique in inhibition of the expression of the mouse fibrinoge......
Silencing the gene encoding C/EBP homologous protein lessens acute brain injury following ischemia/r
C/EBP homologous protein, an important transcription factor during endoplasmic reticulum stress, participates in cell ap......
Colonoscopy sometimes causes pain during insertion,especially in difficult cases.Over-insufflation of air causes elongat......
Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in d
AIM: To investigate the mechanism of interleukin (IL)-6 secretion through blocking the IL-17A/IL-17A recepto (IL-17RA) s......
Identification of cellular genes showing differential expression associated with hepatitis B virus i
阜阳市局 ￡4a 局长——_MINtkMMtgj’＃lnH＄B——q.M——D收束志不他只邀狲位J则【科校为先导推动地租工作.B为广为戳民U用凶盼引眯B.......
Background The relationship between signal transduction and tumors has become one of the foci in cancer research. Signal......
Impact of Pitx3 gene knockdown on glial cell line-derived neurotrophic factor transcriptional activi
Pitx3 is strongly associated with the phenotype, differentiation, and survival of dopaminergic neurons. The relationship......
Aim:To study the role of acid-sensing ion channel (ASIC) 1a in the cell death andapoptosis induced by extracellular aci......
在当今基因干预和基因治疗领域里,有两项极为引人注目的技术,那就是反义核酸技术和ribozyme技术。而二者的完美结合,则成为基因治......
AIM: To study the effectiveness and mechanisms of anti-human vascular endothelial growth factor (hVEGF) hairpin ribozyme......
Protein inhibitor of activated STAT 4(PIAS4) regulates liver fibrosis through modulating SMAD3 activ
Excessive fibrogenesis disrupts normal liver structure,impairs liver function,and precipitates the development of cirrho......
促卵泡激素(FSH)在灵长类精子发生中具有重要作用,但非第二性征所必需;可考虑作为避孕疫苗的靶抗原。但75％的抗FSH血清有抗黄体生......
鎯头核酶(hammer ribozymes)在细胞内不能抑制乙型肝炎病毒(HBV)复制,所以本文对发夹环核酶(hairpin ribozymes)进行了研究。首先......
Growth inhibition induced by short hairpin RNA to silence survivin gene in human pancreatic cancer c
BACKGROUND:Survivin is known to be overexpressed in various human malignancies,including pancreatic cancer,and mediates ......
To study the expression activity of various vectors containing anti-caspase-3 ribozyme cassettes in vivo, and to further......
To study the preparation and cleavage of hairpin ribozyme (HpRz) directed against the transcript of HBV core region in v......
AIM:To explore whether HDV ribozymes have the abilityto trans-cleave HCV RNA.METHODS:Three HDV genomic ribozymes werede......
AIM:To study the preparation and cleavage activity of HpRzdirected against the transcript of HBV core gene in vitro.MET......
The 60mer oligonucleotide microarray was designed and applied to detecting of SARS (severe acute res-piratory syndrome) ......
The application of a new fluorogenic probe-based PCR assay (PCR duplex scorpion primer assay) to the detec-tion of Hepat......
Inhibition of human La protein by RNA interference downregulates hepatitis B virus mRNA in 2.2.15 ce
AIM:To investigate the role of human La protein in HBVmRNA expression.METHODS:Three human La protein (hLa) specific siR......
AIM: To study the function of α-fetoprotein (AFP) in SMMC-7721 hepatoma cells. METHODS: A hairpin siRNA expressing plas......
AIM: To study the effect of short hairpin RNAs (shRNAs) expressed from DNA vector on hTERT expression. METHODS: Oligonuc......
Activity identification of ribozyme and U1 snRNA chimeric ribozyme against TGFβ1 in cell-free system
Transforming growth factor? (TGFβ1) is known to be intimately involved in many cellular processes. To explore the mecha......
Background RNA interference using short hairpin RNA (shRNA) can mediate sequence-specific inhibition of gene expression ......
The discovery of microRNAs (miRNAs) is a remarkable breakthrough in the field of molecular genetics, as miRNAs are key a......
Expression of aquaporin-1 in rat pleural mesothelial cells and its specific inhibition by RNA interf
Background The discovery of water channel aquaporins(AQPs)has greatly expanded the understanding of theregulation of th......
Transiently Expressed Short Hairpin RNA Targeting 126 kDa Protein of Tobacco Mosaic Virus Interferes
RNA-interference (RNAi) silences gene expression by’guiding mRNA degradation in asequence-specific fashion.Small inter......
To study the effect of specific G11 nucleotide on cleavage activity of hairpin ribozyme in vitro, the 32P-labeled pKC t......
RNA interference blocking the apoptosis in HEK293 cells induced by overexpression of alpha-synuclein
BACKGROUND:Overexpression ofα-synuclein can induce cell apoptosis.RNA interference(RNAi) may block specific gene functi......
In order to investigate the effects of vector-based hairpin small interference RNA(shRNA) on the reversal of multi-drug ......
Liver-specific Reduction of Mfn2 Protein by RNAi Results in Impaired Glycometabolism and Lipid Homeo
Mitofusin-2 (Mfn2) gene expression is positively correlated with insulin sensitivity in patients with type 2 diabetes. H......
