Regulatory role of evodiamine (EVO) on bone marrow derived dendritic cell (BMDC) in vitro

来源 :中国细胞生物学学会2015年全国学术大会 | 被引量 : 0次 | 上传用户:lzy9061
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  Objective To explore the influence of EVO on BMDC induced by lipopolysaccharide (LPS) in vitro.Methods C57BL/6 mice with the age range of 4-6 weeks were used in the study and bone marrow precursor cells in their femurs were separated and incubated with RPMI1640 and 10% FBS under the circumstance of 37℃ and 5% CO2.During this time, GM-CSF (25ng/mL)were added every 48h in order to induce BMDC.
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