【摘 要】
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The male reproductive toxicity of endosulfan has been proved.However, the molecular mechanisms underlying the apoptosis effects of endosulfan in spermatogenic cells remain poorly understood.To investi
【机 构】
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Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital Medical University
【出 处】
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2016(第二届)毒性测试替代方法与转化毒理学(国际)学术研讨会暨有害结局路径(AOP)与风险评估培训会议
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The male reproductive toxicity of endosulfan has been proved.However, the molecular mechanisms underlying the apoptosis effects of endosulfan in spermatogenic cells remain poorly understood.To investigate the mechanism of reproductive toxicity indced by endosulfan, 32 wistar male rats were randomly and averagely divided into four groups, and received 0, 1, 5 and 10 mg/kg/day endosulfan for a period of 21 days, respectively.GC-1 spermatogenic cells line were divided into four groups, and exposed to 0, 6, 12,24μg/ml endosulfan for 24 hours, respectively.The results showed that endosulfan decreased the cell viability, damaged cell membrane and induced the apoptosis in spermatogenic cell; obviouly activated the protein expression of PKC-δ, p53, p21cip1, p271ip1, Fas, FasL, Caspase-8, Caspase-3,while inhibited the expression of E2F-1; endosulfan also induced oxidative stress and DNA damage in spermatogenic cells.Endosulfan could lead to the apoptosis of spermatogenic cells by activating the negative regulation factors of cell cycle resulting in E2F-1-induced apoptosis, on the other hand, endosulfan might cause the apoptosis by death receptor pathway resulting from oxidative stress.
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