Purpose: We tried to develop a formulation for local anesthetic with quicker onset.These formulations would be applied in laser-assisted surgery.Methods: The hot pre-emulsion is then processed in a temperature controlled high pressure homogenizer.The obtained nanoemulsion recrystallizes upon cooling down to room temperature.In vitro release studies were performed with flow-through Franz diffusion cells.Results: The phase diagram was constructed by titration of homogenous liquid mixtures of Compritol 888 ATO, surfactant (Span 60), with water at room temperature.The solid lipid nanoparticles were formulated with 2.5% lidocaine and prilocaine.The penetration of lidocaine from these formulations increased with the melting point of lipid in these lipid particles.Furthermore, the results demonstrate that the penetration of lidocaine or prilocaine in formulation containing Brij 30 or Brij 35 is higher than that containing Span 60.Consulsion: The solid lipid nanoparticles composed of Compritol 888 ATO and Brij 35 were the best formulation to improve quicker penetration for lodocaine and prilocaine in vitro tests.These results would supply the surgeon to develop new therapeutic approaches in laser-assisted surgery.