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A new mosquitocidal gene,cyt1Aa6,was isolated and cloned from the novel Bacillus thuringiensis strain LLP29,previously isolated from the phylloplane of Magnolia denudata.Nucleotide sequence analysis of cyt1Aa6 indicated that the open reading frame consisted of 750 base pairs,encoding 249 amino acid sequences with a calculated molecular weight of 27.3681 kDa and a PI value of 4.77.