Aging is a complex and universal phenomenon.In the last few decades,application of molecular genetics to the study of aging in model organisms has revealed genes/pathways with conserved effect on lifespan across species.However,the molecular causes of aging and death at the cellular level remain elusive.In the past few years,we have used systems biology approaches to tackle aging in the single cell organism yeast.We have developed a flow cytometer based assay to measure the asymmetry protein distribution during cell division.We have developed microfluidic systems that allow us to directly observe the process of aging at the molecular level in single cells throughout their lifespan.We used RNA-seq and ribosomal footprinting to characterize the molecular mechanism of Calorie Restriction which extends lifespan in many different organisms.Combined with various functional genomics tools,we aim to reconstruct the global gene regulatory networks for aging.