【摘 要】
：
Seeds per silique(SS)is one of the three important components of yield in oilseed rape(Brassica napus L.); however,the genetic architecture of this trait remains largely unclear.In this study,a F1 pla
【机 构】
：
Huazhong Agricultural University,Wuhan,430070,China
【出 处】
：
第十九届国际十字花科遗传学会及芸薹属2014年年会
论文部分内容阅读
Seeds per silique(SS)is one of the three important components of yield in oilseed rape(Brassica napus L.); however,the genetic architecture of this trait remains largely unclear.In this study,a F1 plant derived from two inbred lines differing in seeds per silique were used to generate 180 double haploid lines.QTL scanning based on this DH population indicated that a major QTL,qSS.C9,was located on the C9 chromosome.Near-isogenic lines(BC3F2)for qSS.C9 was then produced by successively backcrossing the high SS parent Y106 with the recurrent parent HZ396.A total of 152 BC3F2 plants and their parents were evaluated for SS.A Chi-square test showed that the ratio of the frequency distribution follows a the Mendelian segregation ratio of 3:1(x2= 0.355,P>0.05),suggesting that only one locus is responsible for the SS variation in the BC3F2 population.QTL analysis further confirmed that qSS.C9 explaining 85.8%of phenotypic variance with additive and dominant effects of 6.1 and 5.7 SS,respectively,was located between the markers SRC9-022 and SCC9-005.The other two SSR markers(SRC9-27 and SRC9-298)closely linked to qSS.C9 were identified by using high-SS and low-SS bulks,and further explored to precisely map the qSS.C9 by assaying 2,058 extremely low SS plants from a BC4F2 population.Comparative mapping between B.napus,B.rapa and B.oleracea showed that the qSS.C9 candidate region restricted by SRC9-27 and SRC9-298 corresponds to a 140 kb physical interval on B.rapa chromosome A10 but a 267kb physical interval on B.oleracea chromosome C9.According to the B.oleracea Database,fourty-two coding genes were predicted in this region.Comparative sequence analysis between parental lines showed that two genes would be likely the candidate genes of qSS.C9.
其他文献
Sclerotinia rot (SR),caused by Sclerotinia sclerotiorum,is a serious threat to oilseed Brassica production in Asia,Europe,North America and Australia and to vegetable crucifer production worldwide.Man
Meeting the rapidly growing global demand for vegetable oil from oilseed rape/canola (Brassica napus) is being increasingly threatened by dry conditions in many parts of the world.Brassica rapa,one of
In this study,we investigated eight injured varieties among thirty four oilseed rapes and analyzed their index of bird pest,compared glucosinolates content with injured and normal varieties,expecting
Abiotic stresses,such as drought,lead to crop growth damage and a decrease in crop yields.Stomata control CO2 uptake and optimize water use efficiency,thereby playing crucial roles in abiotic stress t
The superiority of breeding approaches and advancement of breeding technology through CHA (Chemical Hybridization Agent)inducing male sterility to select rapeseed hybrids have been gradually recognize
A random amplified polymorphic DNA(RAPD)molecular marker linked to late-bolting gene in pak-choi(Brassica campestris ssp.chinensis Makino L.)Bulked segregant analysis(BSA)and random amplified polymorp
Clubroot disease has gained great relevance in all major oilseed rape cropping areas.Host resistance is the most important clubroot control method;however,most resistance sources are race-dependent an
Nowadays Oilseed Brassica crops still play a key role in oil production worldwide.This is mainly due to the impact of breeding efforts devoted to significant traits of agronomical and economical inter
Many crop seed traits such as seed weight,oil content etc.are formed in the embryonic development,which are also the results of the interaction with the maternal plant.Until now,for these traits,more
Although seed weight(SW)and silique length(SL)are important determinants of yield potential in rapeseed(Brassica napus L.),their genetic basis have been poorly understood.To dissect the genetic basis