【摘 要】
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Enrichment and purification of silymarin from Silybum marianum extracts were performed using five types of macroporous resins.Static tests indicated that HPD-600 resin had the best performance.To opti
【出 处】
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The 9th Asian Biophysics Association Symposium (ABA2015)(第九届
论文部分内容阅读
Enrichment and purification of silymarin from Silybum marianum extracts were performed using five types of macroporous resins.Static tests indicated that HPD-600 resin had the best performance.To optimize the separation process, dynamic adsorption and desorption tests were carried out.The optimal adsorption parameters were as follows: initial silymarin concentration in the sample, 7.64 mg/mL;pH, 5.0;sample loading, 2.3 bed volume (BV);flow rate, 2 BV/h;and temperature, 25℃.The optimal desorption parameters were: deionized water and 20% ethanol, 5 BV each;60% ethanol, 10 BV;and flow rate, 2 BV/h.After HPD-600 purification, silymarin content in the extract increased from 11.38% to 53.41%, and the recovery yield was 82.24%.The results demonstrate that HPD-600 resin can efficiently concentrate silymarin in the S.marianum extracts and may also be used for silymarin isolation from other plants.
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