CD73与乳腺癌干细胞相关性的初步研究

来源 :中国病理生理学会第十四届肿瘤专业委员会、第十五届免疫专业委员会联合学术会议 | 被引量 : 0次 | 上传用户:Truth_Tiger
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肿瘤细胞中存在具备干细胞特点的细胞群,它们可以无限增殖、自我更新并分化为各种恶性程度的肿瘤细胞,从而导致肿瘤细胞不断产生、生长、转移、耐药并复发.目前在多种肿瘤细胞中检测并分离获得了肿瘤干细胞样细胞.CD73是AMP生成腺苷的关键酶,本课题组的前期实验及相关文献证明,CD73促进乳腺癌的发生、发展.但CD73与乳腺癌干细胞的相关性还未阐明。 目的:本研究拟从乳腺癌干细胞方面继续深入探讨CD73促进乳腺癌发生发展的机制。 方法:CD44+CD24-和乙醛脱氢酶1(ALDHI)是乳腺癌干细胞常用的鉴别标志。利用流式细胞仪检测多种乳腺癌细胞株中ALDH1+和CD44+CD24细胞的比例,并观察CD73干扰和过表达对这些细胞亚群比例的影响,最后利用悬浮球培养法富集了乳腺癌干细胞,并利用实时PCR和Western Blot比较在乳腺癌干细胞球与正常培养细胞株之间CD73表达的差异。 结果:流式细胞仪检测发现,MCF-7细胞中可以检测出CD44十CD24一细胞群,并且感染CD73过表达病毒的MCF-7细胞中CD44+CD24一细胞比例较对照组明显提高(P<0.05)。 结论:CD73在乳腺癌干细胞中表达增高,并可提高乳腺癌细胞中干细胞亚群的比例。提示,CD73与乳腺癌干细胞的正相关可能是其促进乳腺癌发生发展的机制之一。
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