【摘 要】
：
Neuron-specific enolase (NSE) is the key molecular marker for diffuse neuroendocrine system (DNES) cells,its expression in the pregnant corpus luteum (CL) of dairy goats was studied by the immunofluor
【机 构】
：
College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, China
【出 处】
：
中国畜牧兽医学会动物解剖学及组织胚胎学分会第十七次学术研讨会
论文部分内容阅读
Neuron-specific enolase (NSE) is the key molecular marker for diffuse neuroendocrine system (DNES) cells,its expression in the pregnant corpus luteum (CL) of dairy goats was studied by the immunofluorescence method and the ultra structural characteristics of luteal cells were detected by the electron microscopy to identify the existence of DNES cells in the pregnant CL of dairy goats.Besides,the coding sequence of dairy goats NSE gene was cloned and its biological information was analyzed.Results revealed that NSE immunopositive cells exhibited widespread cytoplasmic staining throughout the whole pregnant CL.In addition,these cells showed typical characteristics of DNES cells in the electron microscopy.These results suggested that many DNES cells exist in the pregnant CL of dairy goats.Meanwhile,we identified the coding sequence of dairy goats NSE (GenBank accession No.JN887466).Its nucleotide sequence homology was found to be 97.9％,89.3％,90％ and 92.6％,respectively,compared with that of Bos taurus,Rattus norvegicus,Mus musculus and Homo sapiens,while the amino acid sequence homology was 99.1％,97％,97.2％ and 98.2％ respectively.These results first showed that the functional amino acids coded by the NSE gene were highly conserved in Caprine,Bos taurus,Rattus norvegicus,Mus musculus and Homo sapiens.It was implied that the gene NSE in dairy goats had close homology to that of NSE of other species.Our findings demonstrated the possible existence of DNES cells in pregnant CL,providing new clue for further understanding of interactions between the neuroendocrine and reproductive system.Characterization of gene sequence of dairy goats NSE will enable us to synthesize interference RNA for further study on the role of NSE on the formation,function and apotosis of pregnant CL in dairy goats.
其他文献
Glucagon is the hyperglycemic factor that opposes the action of insulin.It stimulates the breakdown of liver glycogen and increased glucogeogenesis.Glucagon is located in the tissues of the pancreas o
鲤鱼作为人类一种重要的动物蛋白来源,是世界淡水养殖业中一种非常重要的鱼类.尽管鲤鱼在农业生产中的地位和产量近年来有所提高,但是对其发育机制研究还比较匮乏.肌肉是脊椎动物的一种重要组织,广泛分布在各组织器官及骨骼表面.骨骼肌又称横纹肌,由具有收缩能力的肌细胞组成,由结缔组织覆盖并接合在一起.是支持脊椎动物运动功能的重要组织.人们摄取的鲤鱼蛋白也主要来源于骨骼肌组织.
为了探明小鼠Desmin基因启动子在体细胞中的表达活性,本研究根据NCBI中小鼠Desmin启动子的序列,利用生物软件Primer Premier 6.0设计序列上、下游引物,克隆小鼠基因组中Desmin启动子片段,然后与pAcGFP1- N1载体中的CMV启动子进行置换以构建真核表达载体pAcGFP1 -N1 -Des,然后将pAcGFP1- N1- Des依次转染CHO- K1、COS-7、2
研究骨髓间充质干细胞(BMSCs)向雪旺细胞诱导分化的能力,为组织工程神经选择一种理想的种子细胞.采用percoll密度梯度法分离骨髓间充质干细胞.流式细胞仪检测干细胞表面特异MARK CD29、CD45、CD80、CD90评价干细胞的生物学特性.采用化学诱导和共培养诱导两种方式诱导骨髓间充质干细胞向雪旺细胞分化,观察诱导后细胞的形态变化.采用免疫荧光染色检测雪旺细胞特异性标记物S-100和GFA
Interleukin-21(IL-21) was recently discovered using a functional cloning approach based on expression of its receptor,has been found it influences proliferation of T and B cells and cytolytic activity
To investigate IL-21 distribution characteristics and development changes in silkie immune organs,in order to breeding and provide the theoretical basis for disease control and prevention.The paraffin
The present study was performed to investigate the morphology and topographic distribution,developmental changes of Somatostatin-producing cells in the small intestinal of African ostrich chicks at di
Ultrastructural characteristics of different subtypes of interstitial cells of Cajal (ICC) remain unclear in birds however birds have significant economical and scientific notability.Our aim was desc
The alpha melanocyte stimulating hormone receptor (MC1R) is one of five G-protein coupled receptors belonging to the melanocortin subfamily,mclr gene has been known to play a major role in regulating