【摘 要】
：
In order to obtain a certain length(630bp) of constant domain gene, a pair of primer was designed according to the conserved nucleotide sequence of gallus(EU477527.1) and Taeniopygia guttata (XM_00218
【出 处】
：
中国免疫学会第九届全国免疫学学术大会
论文部分内容阅读
In order to obtain a certain length(630bp) of constant domain gene, a pair of primer was designed according to the conserved nucleotide sequence of gallus(EU477527.1) and Taeniopygia guttata (XM_002189232.1) IFNAR-1 gene fragment that was published by GenBank.Special primers were designed by the Race method to amplify the 3terminal cDNA.A pair of primer was designed according to the conserved nucleotide sequence of 630bp IFNAR-1 fragment.A certain length(274bp) of fragment was obtained.The fragment was cloned by Peasy-T1 and amplified by relative quantitation PCR with SYBR Green Ⅰ dye.
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