Efficient kinetic resolution of phenyl glycidyl ether by a novel epoxide hydrolase from Tsukamurella

来源 :2015年上海市研究生学术论坛(生物化工) | 被引量 : 0次 | 上传用户:e56urty
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  Enantioselective hydrolysis of racemic epoxides mediated by epoxide hydrolases (EHs) is one of the most promising approaches to obtain enantiopure epoxides.In this study, we identified and characterized a novel EH (TpEH1) from Tsukamurella paurometabola by analyzing the conserved catalytic residues of EH.TpEH1 was overexpressed and purified, and its catalytic properties were studied using racemic phenyl glycidyl ether (PGE) and its derivatives as substrates.
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