【摘 要】
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The complete A+T-rich region of mitochondrial DNA has been cloned and sequenced from Antheraea pernyi (Lepidoptera: Saturniidae).Nine haplotypes were obtained from 16 inbred strains of the domesticate
【机 构】
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辽宁省昆虫资源工程技术研究中心,生物科学与技术学院,沈阳农业大学,沈阳 110866 辽宁省昆虫资
【出 处】
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第十届家(柞)蚕遗传育种及良种繁育学术研讨会
论文部分内容阅读
The complete A+T-rich region of mitochondrial DNA has been cloned and sequenced from Antheraea pernyi (Lepidoptera: Saturniidae).Nine haplotypes were obtained from 16 inbred strains of the domesticated type with the range from 550 to 554 bp in size,exhibiting a low degree of sequence divergence from 0.18 to 0.91%.Two haplotypes were observed from three individuals of the wild type with 516 or 517 bp,respectively,showing a length polymorphism and a relatively high degree of sequence divergence compared with those of the domesticated type.Phylogenetic analysis showed the presence of two clearly differentiated mitochondrial clades: the domesticated type and the wild type.The length polymorphism between the two types was caused by the presence of a variable number of tandemly repeated units.Several conserved structural elements previously described in other insects were also present in the A.pernyi A+T-rich region.Moreover,two tRNA-like structures were observed in this region.
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