In order to study the effect on inducing male sterility of chemical hybridizing agent SX-1 on Brassica napus L.in Zhangye area and screen out the optimal concentration and period for spraying,so as to lay the foundation for breeding of chemical induction male sterile,production of hybrid seeds and generalization,the experiment was carried out from 2009 to 2013.Combining the climatope of Zhangye area,different concentrations of chemical hybridizing agent SX-1 were sprayed for two times on YD219A、YD2013 et al.and some excellent inbred lines for inducing male sterility.