The conventional cell culture method has been driven from the use of petridish to the well plates in order to reduce the amount of culture media.Combined with microtechnology,the localized culture of cell onto the specific position by using of stencil [1],microcontact printing,photolithography,micromolding,dip-pen spotting,and inkjet printing allowed a precise control of the size,shape and location of the cultured cell into micropatterns.The localized,and micropatterned culture provides many number of cell samples for the subsequent analysis which are cultured at the same condition.However the patterned cells are immobilized on the surface,therefore the limitation still exists that there are difficulties on the cell analysis of the separated cell patterns.A separable silicon microplate based on silicon fabrication process on SOI wafer has been proposed [2].The cell were cultured on the mobile microplates,therefore manual handling of multiple number of cell patterns were possible.However the fabrication involved many complex steps of photolithography,ICP-RIE,and wet etching.In this study,we propose another type of microplates which are fabricated by single process of photholithography using hydrogel.The process are simple,low-cost,and does not require expensive MEMS facilities.The movable microplates were applied onto the culture of NIH/3T3 cells.Because the fabricated microplates are detachable,it provides efficient way for further studies of cell biology by allowing separated analysis of many number of cell samples which are cultured at the same environment with small amount of cell media.