The unusual properties of metal-organic frameworks (MOFs) such as permanent nanoscale porosity, high surface area, uniform structured cavities and the availability of in-pore functionality and outer-surface modification are advantageous for diverse applications.1 Here we present our recent research on the application of MOFs for analytical separation.2-9 Examples include: (a) Exploration of coordination polymer as sorbent for flow injection solid phase extraction on-line coupled with high-performance liquid chromatography for determination of polycyclic aromatic hydrocarbons in environmental materials; (b) In situ hydrothermal growth of MOF-199 films on stainless steel fiber for solid-phase microextration of gaseous benzene homologues; (c) MOF-5 as sorbent for in-field sampling and preconcentration in combination with thermal desorption GC-MS for determination of atmospheric formaldehyde; (d) MIL-101 for high-resolution gas-chromatographic separation of xylene isomers and ethylbenzene; (e) ZIF-8 nanocrystals coated capillary for molecular sieving of linear alkanes from branched alkanes along with high-resolution gas-chromatographic separation of linear alkanes; (f) Fabrication of IRMOFs coated capillary columns for high-resolution gas-chromatographic separation of persistent organic pollutants; (g) MOFs based tandem molecular sieve as a dual platform for selective SPME and high-resolution gas-chromatographic separation of n-alkanes in complex matrices; (h) MOFs for efficient enrichment of low-abundance peptides with simultaneous exclusion of high-abundance proteins from complex biological samples; (i) MOFs for high-performance liquid chromatography.