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<正>A new quantification method using high performance liquid chromatography- tandem mass spectrometry（HPLC-MS） method was developed and validated for simultaneous determination of 15 major components,namely adenosine,scopoletin,xanthotoxol,xanthotoxin,psoralen,isoimpinellin,bergapten, oxypeucedanin,imperatorin,cnidilin,isoimperatorin,chlorogenic acid,caffeic acid,vanillic acid and ferulic acid in Radix Glehniae,an important traditional Chinese medicine.The chromatographic separation was performed on an Agilent Zorbax Eclipse XDB-C18 column with isocratic elution consisting of 0.1%formic acid and methanol（30:70,v/v） at a flow rate of 1.0ml/min.Detection was carried out on a triple quadrupole tandem mass spectrometer by multiple-reaction monitoring（MRM） and an electrospray ionization source was operated in negative mode and positive mode at the same time.All calibration curves showed good linearity（r2>0.997） within test ranges.The reproducibility was evaluated by intra- and inter-day assays and R.S.D.values were less than 5.0%.The recoveries were between 94.9%and 104.8%.The method was applied to the determination of 15 constituents in 20 samples of Radix Glehniae from different sources and the results indicated that the developed HPLC-MS/MS method was a simple,rapid and reliable method for the quality evaluation of Radix Glehniae.