树鼩IL-2基因序列的克隆及分子特征分析

来源 :首届昆明灵长类动物模型学术论坛 | 被引量 : 0次 | 上传用户：shepuqi4709

【摘要】

：

树鼩作为人类疾病良好模型己受到广泛关注,但对其白细胞介素一2方面的研究尚属空白.本实验以培养的树鼩淋巴细胞总RNA为材料,通过RT-PCR技术克隆出长度约为465bp的树鼩IL-2完

【作者】

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黄晓燕代解杰李明利徐娟高跃东王文广殷安国李晓飞孙晓梅

【机构】

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中国医学科学院/北京协和医学院医学生物学研究所,云南省重大传染病疫苗研发重点实验室,云南,昆明,650118中国科学院昆明动物研究所,动物模型与人类疾病机理重点实验室,免疫生物学实验室,云南,昆明,6

【出处】

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首届昆明灵长类动物模型学术论坛

【发表日期】

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2012年11期

【关键词】

：

Tree shrews Interleukin 2 Cloning Structure Function

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论文部分内容阅读

树鼩作为人类疾病良好模型己受到广泛关注,但对其白细胞介素一2方面的研究尚属空白.本实验以培养的树鼩淋巴细胞总RNA为材料,通过RT-PCR技术克隆出长度约为465bp的树鼩IL-2完整开放阅读框(ORF),并通过Clustal W等软件对其序列和分子特征进行了分析,发现树鼩IL-2 cDNA编码一个由154个氨基酸组成的蛋白质,采用MEGA5.0构建进化树发现,树鼩与人、恒河猴亲缘关系较近.

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树鼩IL-2基因序列的克隆及分子特征分析

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