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This paper has determined the effects of different UV inactivation time on the lethal rate of protoplast,and effect of different drugs marker.We know that medium with 0.1%or 0.01%three triadimefon fungicides has inhibitory effect on the growth of 2.1492.but does not work with 1OO1.when the 1001 is beamed by ultraviolet lamp for 120 seconds.