小麦近缘植物A、S、D基因组α-醇溶蛋白基因的克隆及序列分析

来源 :第四届全国小麦基因组学及分子育种大会 | 被引量 : 0次 | 上传用户:waxizhaojing
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α-醇溶蛋白是小麦种子中含量最高的醇溶蛋白,同时也是引起人体乳糜泻病的主要蛋白.本实验以来源于小麦近缘种栽培一粒小麦(AA)、拟斯卑尔脱山羊草(SS)、粗山羊草(DD)材料DNA为模板,用小麦种子醇溶蛋白保守引物进行PCR扩增,共获得23个α-醇溶蛋白基因,GenBank登录号分别为:KC421073-KC421095,编码279-318个氨基酸.
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