The precision of orbit determination for navigation satellites with regional distributed stations may be improve by introducing on-board GNSS observation data.The method of orbit determination combining the on-board data of LEO with the observation data of regional monitor stations is discussed.In order to validate this proposed approach and its performance,The observation data of 7 GPS stations in China and on-board data of GRACE-A&B during March 16th~31th 2011 were collected and orbit determination experiments were carried out.The result shows that,compared with the result of 7 stations,the visibility of GPS satellites is increased by about 14%,meanwhile,the precision of GPS orbit expressed in RTN components are improved by about 35%,44% and 45% respectively when introducing only GRACE-B,improvement in RTN components of 51%,60% and 62% are achieved when both GRACE-A and GRACE-B are introduced and the visibility of GPS satellites is increased by 18% in this case.The proposed approach will provide a new idea to improve the orbit determination accuracy of navigation satellite under the restriction of regional monitoring stations.