Bioprinting offers huge potential to regenerate biomimetic three-dimensional(3D)tissue,that can be used to regenerate damaged tissue and provide platforms for drug screening.However,the current polymeric bioinks are poorly conductive,which delay efficient electrical cell coupling while printing live 3D cardiac tissues.To solve this problem,a conductive gold nanorod(GNR)-incorporated bioink is developed for printing 3D functional cardiac tissue constructs.At optimized concentrations of GNR,the conductive bioink allows for the easy integration of induced pluropotent stem cells(iPSCs)-derived cardiomyocyte with hydrogel.As a result,rapid deposition of cell-laden fibers at a high resolution is possible,while reducing shear stress on the encapsulated cells.In the printed cardiac tissue constructs,the incorporated GNRs bridge the electrically resistant pore walls of polymers,improve the cell-to-cell coupling,and promote synchronized contraction of the bioprinted constructs.After being implantated on the infarcted myocardium,the 3D printed cardiac tissue could integrated with host myocardium and improve the heart function significantly.Given its advantageous properties,this 3D printed conductive cardiac tissue may find wide application in ischemic heart disease.