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As early as in 1851, Addison reported that adrenal steroid hormones are vital for human health.As everyone knows, the secretion of adrenal steroid hormones in stressed body will increase.This increase will destroy the metabolizability of water, electrolyte, saccharide etc.And then all kinds of psychopathies are likely to be induced.Therefore, it is very important and highly desirable for clinical analysis to determine the adrenal steroid hormones in human body.So far, various methods such as high performance liquid chromatography (HPLC) and radioimmunoassay (RIA) have been reported.Chun-Hsueh Wu et al reported on the determination of corticosterone in mouse plasma by micellar electrokinetic chromatography(MEKC).