Objective Recently laparoendoscopic single-site surgery is widely performed through transumbilical incision in appendectomy and cholecystectmy, but the practice in the more complex colorectal resection is challenging.Here, Nanfang Hospital Southern Medical University report five patients with diagnosis of rectosigmoid cancer subjective to laparoendoscopic single-site (LESS) colorectomy through a pfannestiel incision with a glove access.Methods LESS colorectomy was performed through a Pfannestiel incision on each patient with diagnosis of rectosigmoid cancer.A 3-5 cm transverse Pfannestiel incision was made on the skin with pubic hair 3-4 cm above the pubic symphysis followed by a self-made glove access was constructed by inserting a soft tissue retractor and a surgical glove.High ligation of inferior mesenteric vessel and intracorporeal anastomosis were done.5 cases were included in this study.Each operation was completed with common laparoscopic instruments.Results The 5 procedures were completed successfully without intraoperative complications and conversions at 205, 68, 217, 115, and 108 minutes.The estimated blood losses were 20, 10, 10, 50, and 20 ml respectively.The number of lymph node harvested was 12, 13, 15, 19, and 16.All distal tumor-free margins exceeded 5 cm.They were discharged without recorded complications.In the 1-year follow-up data, one was dead for pulmonary embolism in postoperative month 11, while other four were confirmed no recurrence and metastasis.Conclusions LESS colorecotmy performed above the pubic symphysis through a homemade glove access is safe and technically feasible, which can achieve the oncologic radical cure for rectosigmoid cancer.It possesses not only good minimally invasive advantages but also excellent cosmetic effects as well.