Alpinia katsumadai hayata is a pungent taste herbal used for the treatment of metabolic dysfunctions.To address the chemical basis and molecular mechanism responsible for its role in lipid metabolism,thirteen major chemicals were assessed by high content screening in differentiated 3T3-L1 adipocytes.Cardamonin exhibited dose–dependent anti-adipogenic effect and the IC50 value was defined as 0.84 μM.Moreover,immunofluorescence labelling revealed that CDN appreciably decreased the expression of adipocyte-specific biomarkers.Namely,proliferator-activated receptor gamma(PPAR?)and CCAAT enhancer binding protein α(C/EBPα)(P < 0.001).Indirectly,fatty acid synthase(FAS)that in charge of free fatty acid production,was significantly reduced.Interestingly,the biomarker of brown adipocytes,uncoupling protein-1(UCP-1),was down-regulated(P<0.001).To further illustrate the relationship of cardamonin on regulating fat metabolism,a map of Protein-Protein Interaction was created based on the identified biomarkers through in vitro experiments and enriched proteins.The network suggested potential of cardamonin in thermo sensing through targeting at TRPA1,UCP-1,and FOS.Our study states that cardamonin is the major bioactive chemical ingredient of Alpinia katsumadai hayata.The protein interaction network increases the understanding of molecular mechanism for cardamonin on regulating thermo sensing in adipocytes.