Cyprinid herpesvirus 2 (CyHV-2) is an emerging pathogen in the commercially exploited fish, gibel carp (Carassius auratus gibelio), which has caused huge economic loss in China and appears to be spreading worldwide.In this article, CyHV-2 infection of gibel carp was confirmed for the first time by polymerase chain reaction (PCR), which gave positive results from the tissue samples dissected from moribund fish including gill, kidney, spleen and liver.