【摘 要】
：
To obtain natural or "me-better" antibodies (e.g., affinity-maturated antibodies), phage display libraries are widely used.However, the likelihood of obtaining satisfactory antibodies depends on the l
【机 构】
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Department of Gynecology and Obstetrics, PLA General Hospital, Fuxing Road No.28, Beijing 100853, Ch
【出 处】
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第七届国际分子模拟与信息技术应用学术会议
论文部分内容阅读
To obtain natural or "me-better" antibodies (e.g., affinity-maturated antibodies), phage display libraries are widely used.However, the likelihood of obtaining satisfactory antibodies depends on the library content.Here, we used computer-aided design to model the use of the LoxPS11 site as a linker between the heavy and light variable domains of an antibody for construction of a large single-chain fragment (scFv) antibody phage library by using the Cre/LoxP recombinant system.
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