【摘 要】
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Opaque2 (O2) is a transcription factor that plays important roles during maize endosperm development.Mutation of the O2 gene improves the nutritional value of maize seeds but also confers pleiotropic
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Opaque2 (O2) is a transcription factor that plays important roles during maize endosperm development.Mutation of the O2 gene improves the nutritional value of maize seeds but also confers pleiotropic effects that result in reduced agronomic quality.To reveal the transcriptional regulatory framework of O2,we studied the transcriptome of o2 mutants using RNA sequencing (RNA-Seq) and determined O2 DNA binding targets using chromatin immunoprecipitation coupled to high-throughput sequencing (ChIP-Seq).The RNA-Seq analysis revealed 1605 differentially expressed genes (DEGs) and 383 differentially expressed long,noncoding RNAs.
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