【摘 要】
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Transgene copy number has a great impact on the expression level and stability of exogenous gene in transgenic plants.Quantitative real-time PCR technique, which depends on the use of appropriate endo
【机 构】
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Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University
论文部分内容阅读
Transgene copy number has a great impact on the expression level and stability of exogenous gene in transgenic plants.Quantitative real-time PCR technique, which depends on the use of appropriate endogenous reference genes, has been widely accepted as an accurate and time-saved method in the copy number detection of transgenic plants and GM detection to meet the regulatory and legislative requirement.To date, most transgene crops have been developed corresponding endogenous reference genes, some species have multiple applicable endogenous reference genes, but limited in sugarcane.
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