【摘 要】
：
目的 药物治疗和手术治疗是目前控制癫痫的主要方法,但疗效果远未另人满意.深部脑刺激(DBS)成为控制难治性癫痫的新方法.DBS通常采用高频率电刺激,但是高频率电刺激会干预靶点的生理功能并导致靶点损伤,此外长期高频电刺激有诱发新癫痫灶的危险.最近研究显示低频率(1-3 Hz)电刺激癫痫灶点也可能具有抗癫痫的作用,具有诱人的应用前景.但癫痫灶点以外脑区的低频率电刺激(LFS)是否同样有抗癫痫作用尚有待
【机 构】
：
浙江大学医学院附属第二医院,杭州310009;浙江大学神经科学所,医学院药理学教研室,杭州310058
【出 处】
：
中国神经科学学会第四次会员代表大会暨第七届全国学术会议(The 7th Biennial Meeting and the
论文部分内容阅读
目的 药物治疗和手术治疗是目前控制癫痫的主要方法,但疗效果远未另人满意.深部脑刺激(DBS)成为控制难治性癫痫的新方法.DBS通常采用高频率电刺激,但是高频率电刺激会干预靶点的生理功能并导致靶点损伤,此外长期高频电刺激有诱发新癫痫灶的危险.最近研究显示低频率(1-3 Hz)电刺激癫痫灶点也可能具有抗癫痫的作用,具有诱人的应用前景.但癫痫灶点以外脑区的低频率电刺激(LFS)是否同样有抗癫痫作用尚有待阐明.我们采用大鼠杏仁核电点燃模型,观察低频率电刺激单侧的梨状皮质(PC)、丘脑内背侧核(MD)、下丘脑结节乳头核(TM)和小脑顶核(FN)对点燃过程的作用.方法 250-300 g大鼠麻醉后向右侧基底外侧杏仁核埋植双电极；在不同动物中分别向单侧PC、MD、TM或者FN埋植双电极,实验中通过该电极给予LFS.动物休息7d后开始每天给予杏仁核电极点燃刺激(60 Hz,1 ms,100-300 μA,1s),点燃刺激结束后立即给予不同脑区15 min的LFS(1 Hz,0.1 ms,100-200 μA).根据Racine评分判定癫痫级别(0-5级),通过Powerlab系统记录杏仁核脑电图,测定后放电持续时间(ADD).
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