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Modification of glycosylation is closely related to pathological changes in living organisms.Qualitative and quantitative analysis of abnormal sugar chains in glycoproteins helps us understand more about how diseases happens and diagnose diseases in early stage.MALDI TOF-MS is of importance in glycomics analysis benefiting from its high throughput,high sensitivity and simple operation.But due to the low ionization efficiency of glycans and the demand of more precises quantitative ananlysis of glycans,chemical derivatization of glycans is generally performed.In recent years,stable isotope labeling has proven to be an effective method for the quantitative glycomics analysis,here we present a set of new designed isotope-labeled hydrazide reagents which can derivatize the glycans reducing end by forming stable hydrazone derivatives.Compared with other derivatization methods such as reductive amination and permethylation,following this strategy,the operation process is a very simple spot and analyze process which means that the MALDI TOF-MS analysis can be directly performed without purification after the derivatization.This quantitative method,using the set of reagents(DMQC and DMQC-4d)which exist 4 Da difference between their molecular ion peak on MS,show a 100-fold dynamic linear range with a good linear relationship(R2=0.9998)for the test on maltoheptaose.In order to verify the quantitative analysis of this method in complex samples,serum samples should be analyzed in the future.