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The optimizing prefreezing pretreatment to keep Lentinus edodes microcosmic quality and construction intact was defined.Response surface methodology (RSM) was used to study the effects of different heating & blanching temperature and time on Lentinus edodes quality.By Central composite design,the optimizing prefreezing pretreatment of Lentinus edodes was:heating & blanching temperature 93.61℃,heating & blanching time 2.32 min.