【摘 要】
：
In this study,a DNA vaccine(pTgGST)encoding T.gondii antioxidant glutathione-S-transferase(TgGST)inserted into eukaryotic expression vector pVAX1was constructed and the immune protective efficacy of i
【机 构】
：
南京农业大学动物医学院,江苏,南京 210095
【出 处】
：
中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
论文部分内容阅读
In this study,a DNA vaccine(pTgGST)encoding T.gondii antioxidant glutathione-S-transferase(TgGST)inserted into eukaryotic expression vector pVAX1was constructed and the immune protective efficacy of intramuscular vaccination of mice with pTgGSTwas analyzed.Mice immunized with pTgGST elicited high titers of total IgG,IgG1,IgG2a,IgA and IgM antibodies,while IgE showed no changes.Also,significant cytokine production of IFN-γ,IL-4 and IL-17 was detected in mice immunized with pTgGST,but not TGF-β1.CD8+ T cells subsets and MHC-I molecules showed significant increase in contrast to CD4+ subsets.Immunization with pTgGST significantly prolonged survival time(14 days)after challenge infection with the virulent T.gondii RH strain,compared with the control groups which died within 8 days.These results suggested that TgGST DNA vaccine could trigger strong humoral and cellular responses and induce partial protection against acute toxoplasmosis.
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