【摘 要】
：
CCN2 is a matricellular protein that appears to be important in scar formation.CCN2 mediates the pro-fibrotic effects in hypertrophic scars (HTSs) through an unknown mechanism; however, many activitie
【机 构】
：
Department of Burns and Cutaneous Surgery,Xijing Hospital,Fourth Military Medical University,127 Cha
论文部分内容阅读
CCN2 is a matricellular protein that appears to be important in scar formation.CCN2 mediates the pro-fibrotic effects in hypertrophic scars (HTSs) through an unknown mechanism; however, many activities of CCN2 protein are known to be mediated through direct binding to integrin receptors.In this study, we investigated the role of integrin α v β 3 in differentiation of hypertrophic scar fibroblasts (HTSF) induced by CCN2.First,we compared the levels of integrin α v β 3 between normal skin and hypertrophic scar (HTS)tissues, and found that integrin α v β3 was up-regulated in HTS.Second, we showed that CCN2 induces HTSF differentiation and collagen synthesis at the mRNAand protein levels.Based on these results, the expression of integrin α v β3 is up-regulated by CCN2stimulation during HTSF differentiation.Blockade of integrin α v β 3 prevented both CCN2-induced HTSF differentiation and collagen synthesis.Furthermore, the CCN2-induced increase in contractility of the HTSF in collagen lattices was inhibited by integrin α v β3 blocking antibodies.In conclusion, our study shows that integrin α v β3 contribute to pro-fibrotic CCN2 signaling.Blocking this pathway may be beneficial for the treatment of HTS.
其他文献
Skin scarring, tightly associated with fibrosis, is a wound-healing by which the body attempts to repair itself following injury.Our previous observations have indicated that IL-10 has the inhibitory
目的 建立一种可精确控制烫伤面积的新西兰兔动物模型.方法 32只新西兰兔被随机分为4组,每组8只,根据接触94℃热水时间不同分别为8s组、10 s组、12 s组、14 s组,以热水接触作为致伤原因,持续时间控制烫伤深度,比较各组创面烫伤深度和愈合时间,并经病理证实.在32组新西兰兔中随机选择8只作为3D扫描组,烫伤24 h后应用3D流动式双目三维扫描技术,扫描计算新西兰兔体表面积及烫伤部位占体表总
目的：探讨肥大细胞膜稳定剂色甘酸钠对大鼠大面积烧伤后胃黏膜组织中肥大细胞形态、胃黏膜损伤程度及组织内丙二醛(MDA)和超氧化物歧化酶(SOD)水平的影响.方法： 20只250g成年SD雄性大鼠随机分为4组：A：正常组；B：大面积烧伤模型组(40％,三度烧伤)；C：模型+色甘酸钠25mg/kg组；D：模型+色甘酸钠50mg/kg组.检测大鼠胃黏膜损伤指数(UI)、组胺、胃黏膜SOD活性及MDA含量.
目的：探讨与自体皮浆非接触式共培养诱导自体脂肪干细胞(ADSCs)向表皮细胞分化的可行性.方法：(1)取6例SD大鼠脂肪组织,用酶消化法分离、培养ADSCs,并于培养第2代行流式细胞仪检测CD49d、CD44和CD106等细胞表型,初步鉴定ADSCs； (2)实验分组：①1*1cm2大鼠皮浆+10％FBS DMEM；②1*1cm2大鼠皮浆+EGF(20μg/L)+10％FBS DMEM组；③含EG
目的 观察早期肠道营养对严重烧伤大鼠高代谢的影响.方法 建立烧伤大鼠早期肠道营养与静脉营养模型,实验动物随机分为正常对照组、早期肠道营养组、静脉营养组,分别测定正常对照组及EN组PN组伤后第1天、第3天、第5天、第7天静息能量消耗(REE)、血浆促肾上腺皮质激素(ACTH)、血浆皮质酮(CORT)、血浆儿茶酚胺(CA)、血清IL-1β、IL-6、TNF α浓度变化情况.结果 严重烧伤后REE均呈逐
Background: Bacterial endotoxin lipopolysaccharide (LPS) is a main initiator of sepsis, which, is responsible for inflammatory events and multiorgan dysfunction, could lead to myocardial depression th
CCN2 plays an important role in the patho-genesis of hypertrophic scars (HTSs).Although CCN2 isinvolved in many fibroproliferative events, the CCN2induction signaling pathway in HTSs is yet to be eluc