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Aim: To investigate the antibacterial effect of Tibetan medicine Qiweihonghuashusheng pills in vitro and provide theoretical basis for clinical application.Methods: M-H agar well diffusion method was adopted to research the antibacterial effect of Tibetan medicine Qiweihonghuashusheng pills on three standard strains(Staphylococcus aureus ATCC25923, Escherichia coli ATCC25922,Salmonella CMCC50319) and 20 clinical isolates; the minimal inhibitory concentration (MIC) of sensitive strains were determined by the method of broth twofold dilution.