【摘 要】
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Perennially in cold,Antarctic fish gradually formed a series of cold resistance mechanism of antifreeze.To identify the antifreeze mechanism of Antarctic fish,ZF4 cells were cultured under 18℃ for col
【机 构】
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College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;Key labora
【出 处】
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第十届世界华人虾蟹养殖研讨会暨2016年中国河蟹产业发展高峰论坛
论文部分内容阅读
Perennially in cold,Antarctic fish gradually formed a series of cold resistance mechanism of antifreeze.To identify the antifreeze mechanism of Antarctic fish,ZF4 cells were cultured under 18℃ for cold acclimation,and 28℃ as control.Cells which cultured more than 15 generations were treated under severe cold stress (10℃).Our data showed that cells underwent cold acclimation had a lower level of apoptosis.The results of karyotype analysis illustrated that cells underwent cold acclimation were tend to be tetraploid.And PI staining also confirmed that the DNA content in the cells after acclimation was higher than that of non-acclimated cells.
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