Ssaccharides and their complexes can play many essential roles in biological systems,but they are hard to be analyzed and have led to serious challenges in glycomic studies.To attack the challenges,various methods or approaches have been tried or explored,of which recognition microarray or carbohydrate chip method has been drawing more and more attentions1–5 because it has exhibited many absorbing features such as high throughput but low consumption of analytes and solvent.