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Drynaria roosii(Nakaike)is a traditional Chinese medicinal fern,known as GuSuiBu.The corresponding effective components of naringin/neoeriocitrin share highly similar chemical structure and medicinal function.Our HPLC-MS/MS results showed that the accumulation of naringin/neoeriocitrin depended on specific tissues or ages.However,little was known about the expression patterns of naringin/neoeriocitrin related genes involved in their regulatory pathways.