【摘 要】
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NAC domain transcription factors are important regulators that activate the secondary wall biosynthesis in wood formation.In this work, we investigated the possible functions of a NAC family member Pt
【机 构】
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National Key Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology and Ecolo
【出 处】
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中国林学会林木遗传育种分会第七届全国林木遗传育种学术大会
论文部分内容阅读
NAC domain transcription factors are important regulators that activate the secondary wall biosynthesis in wood formation.In this work, we investigated the possible functions of a NAC family member PtSND2 (SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN2) using chimeric repressor silencing technology.RT-PCR, subcellular localization and transcriptional activation analyses indicated that PtSND2 is a wood-associated transcriptional factor with the predicted transcriptional activation activity, which could be inhibited by the repression domain SRDX (SUPERMAN REPRESSION DOMAIN X) in yeast.Wood formation was severely repressed in transgenic poplar plants overexpressing PtSND2-SRDX.Meanwhile, the secondary cell wall thickness of xylem fiber was restrained, and the contents of cellulose and lignin were obviously decreased in the stems of transgenic plants.Further studies indicated that expressions of a number of wood-associated genes were down-regulated in the stems of transgenic plants.Our results suggest that PtSND2 may play important roles during the secondary growth of stems in poplar.
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