【摘 要】
：
Background Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome.In this study,we identified the viral genes and their correspo
论文部分内容阅读
Background Recently identified porcine circovirus-like virus P1 has the smallest DNA viral genome.In this study,we identified the viral genes and their corresponding mRNA transcripts.Results The RNAs of P1,synthesized in porcine kidney cells,were examined with northern blotting and PCR analyses.Eight virus-specific RNAs were detected.Four mRNAs(open reading frames(ORFs)1,2,4,and 5)are encoded by the viral(-)strand and four(ORFs 3,6,7,and 8)are encoded by the viral(+)strand.All proteins encoded by the ORFs of the P1 virus are less than 50 amino acids in length,except that encoded by ORF1(113 amino acids).Conclusions We show a very complex viral transcription pattern in P1-infected cells.
其他文献
The harm of Salmonella typhimurium(S.typhimurium)to public health mainly by contaminating fresh food and water emphasizes the urgent need for rapid detection te
Pseudorabies virus(PRV)early protein UL31 is a homologue of herpes simplex virus 1(HSV-1)UL31,which is a multifunctional protein and important for HSV-1 inf
本研究从一头进口病死猪病料中分离得到1株革兰氏阳性球菌,通过对其进行细菌形态学、培养特性、生化特性和分子生物学鉴定,确认分离得到的菌株为绿色气球菌.16S rRNA基因序列
在宁夏吴忠、甘肃兰州等地5个奶牛场选择2个月以上不发情的奶牛158例,其中持久黄体70例、卵巢静止88例,所有病牛在试验前均未给任何药物.病牛子宫灌注藿芪灌注液,每头牛每次1
目的：研究副猪嗜血杆菌SC-1株荚膜多糖输出蛋白的表达及其免疫原性。 方法：运用PCR方法扩增副猪嗜血杆菌SC-1株荚膜多糖输出蛋白(Capsularpolysaccharide export protein,CP
针对目前四川、重庆等地区规模化猪场猪群急性猪丹毒和猪瘟混合感染情况及造成的严重损失,采取综合防控措施和科学的用药方法可有效抑制和治疗该病,笔者从流行病学、临床症状
Little is known about the relationships between the dynamic antibodies and successful bioassay with Toxoplasma gondii infection in the ducks.The objective o
10月下旬以来,在重庆、四川等地的技术服务中,多次治疗了规模化猪场出现的以急性为特点的育肥猪急性死亡的病例,这种疾病已给猪场造成了较大的损失,本文介绍了此次疫病的死亡
目的：为研究丹翘液的体外抗炎作用及其抗炎机制. 方法：利用LPS诱导巨噬细胞建立炎症模型,MTT法检测不同浓度丹翘液对RAW264.7细胞活力影响;NO测试盒检测丹翘液对LPS诱导的NO
为了确诊自然感染疑似化脓隐秘杆菌引起的山羊淋巴结炎病例病原,进行了病原菌分离培养、染色镜检、生化试验和16S rRNA鉴定、动物试验、病理组织切片观察.该病原菌染色镜检为