【摘 要】
：
胃癌是严重危害国人健康的消化系统恶性肿瘤.其发病率呈逐年上升趋势,因此研究其相关分子机制,寻找更有效的治疗手段是目前研究的重点之一.TSA属于羟肟酸类组蛋白去乙酰
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胃癌是严重危害国人健康的消化系统恶性肿瘤.其发病率呈逐年上升趋势,因此研究其相关分子机制,寻找更有效的治疗手段是目前研究的重点之一.TSA属于羟肟酸类组蛋白去乙酰化酶抑制剂(HDACi).它可特异性抑制组蛋白去乙酰基酶,抑制肿瘤细胞的增殖、诱导分化或促进凋亡,在肿瘤治疗方面具有良好的应用前景,但是其发挥作用的分子机制仍有待深入研究.葡萄糖调节蛋白78(GRP78)是热休克蛋白70家族的一员,作为一种内质网分子伴侣在蛋白质的折叠和转运过程及内质网应激反应中发挥重要作用.有研究表明,GRP78在胃癌中过表达与胃癌细胞侵袭力增加及患者不良预后密切相关,但是影响其表达调控的因素仍不十分清楚.因此本研究拟观察TSA对胃癌细胞中GRP78表达的影响.目的：研究TSA对胃癌细胞SGC-7901中GRP78基因表达的影响,进一步了解TSA对胃癌细胞影响的分子机制等.方法：采用quantitative RT-PCR和Western Blot方法检测GRP78基因表达水平.结果：quantitative RT-PCR和Western blot结果表明,TSA处理后,胃癌细胞SGC-7901中GRP78 mRNA及蛋白表达水平明显上调.这有可能影响TSA对胃癌的治疗效果；若在TSA处理同时,采用相应策略抑制GRP78表达,有望改善治疗效果,该研究为TSA在胃癌中的作用机制及其在治疗中的应用研究提供了新线索.结论：TSA上调胃癌细胞SGC-7901中GRP78基因的表达.
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