Epithelial-mesenchymal transition (EMT) has been linked to cancer stem-like (CD44+) cell in the prostate cancer (Pca) metastasis.However, the molecular mechanism remains elusive.Here, we found EMT contributed to metastasis in Pca patients failed in androgen deprivation therapy (ADT).Castration TRAMP model also proved Pca treated with ADT promoted EMT with increased CD44+ stem-like cells.Switched CD44+ cell to EMT cell is a key step for luminal Pca cell metastasis.Our results also suggested ADT might go through promoting TGFβ1-CD44 signaling to enhance swift to EMT.