水稻抽穗期基因RFT1和Ghd7功能标记的开发

来源 :中国作物学会2013年学术年会 | 被引量 : 0次 | 上传用户:skgoo1989
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  RFT1和Ghd7是水稻在长日照条件下控制抽穗期的关键基因,其功能标记的开发将有助于2个基因在水稻分子育种中的应用。我们首先对RFT1的珍汕97 (ZS)和密留46(MY)等位基因进行了测序。序列比较表明,和MY等位基因相比,ZS等位基因在第1外显子上有17个单核苷酸多态(SNP)和1个22bp的插入,在第4外显子上有9个SNP,最终预测蛋白序列存在6个氨基酸置换。根据22 bp的插入/缺失变异,我们设计和发展了1个序标位标记,ZS和MY的PCR扩增产物大小分别为274 bp和252 bp,可轻易地在2%琼脂糖凝胶上分辨开来。
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