【摘 要】
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Rooting-zone restriction is a physical and ecological cultivation mode by constraining roots in a limited volume to modulate vegetative and reproductive growth of plants.Typically,field cultivation us
【机 构】
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Department of Plant Science,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai
【出 处】
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2015 International Conference on Fruit Quality Biology (第二届果
论文部分内容阅读
Rooting-zone restriction is a physical and ecological cultivation mode by constraining roots in a limited volume to modulate vegetative and reproductive growth of plants.Typically,field cultivation usually causes extensive root growth,making it difficult to engage the control of the absorption of moisture and nutrients from soil.Contrarily,rooting-zone restriction,a new cultivation technology,has broken through the traditional theory of deep root system with luxuriant leaves.We have studied grape root-zone restriction over twenty years.Results showed that root-zone restriction significantly inhibited the vine growth and improved fruit quality.Unde root-zone restriction,the genes involved in nitrogen uptake and transport were down-regulated in grapevines,led to the lower nitrogen levels in vegetative organs,and further resulted in the lower expression level of genes involved in nitrogen assimilation and the reduction of GS activity.Therefore,root-zone restriction reduced the conversion efficiency of nitrogen from inorganic to organic form,which should play an important role in significantly inhibiting shoot growth in root-restricted grapevines.However,for the berries,it is the concentration alterations of endogenous ABA and CTK under root restriction that led to sugar phloem unloading being enhanced.Meanwhile,the transcriptional expression of both structural genes(PAL,4CL,CHS2,CHS3,CHI1,F3Hs,DFR,LDOX)and regulatory genes(VlmybA 1-1 and VvmybA1)in anthocyanin biosynthesis pathway was up-regulated.As a result,the content of total and individual anthocyanins levels increased in berry skin.For example,25 anthocyanins were identified in berry skin ofKyohogrape under root-zone restriction,with five of them undetectable in the control.Due to the ecological and economical benefits,this technique has been applied in many grape orchards in China and showed a promising potential value in commercial production of grapes.
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