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Cold stress is one of the adverse effects on apple distribution and production.However, the molecular mechanisms underlying apple cold stress tolerance are not clear.In this study, we cloned a novel R2R3 MYB transcription factor, MdMYB5523, and its close homolog MdMYB0970 from Golden Delicious (Malus × domestica).Expression of MdMYB5523 and MdMYB0970 was slightly induced by cold stress.