论文部分内容阅读
Objective Increasing evidence indicates that in vitro neurogenesis is enhanced by hypoxia, and recent studies have shown that Notch1 signaling pathway, a classic pathway regulating neurogenesis, can be activated by hypoxia.The aim of this study was to investigate whether neurogenesis in dentate gyrus (DG) of hippocampus can be promoted by intermittent hypoxia (IH) and the role of Notchl in the hippocampal neurogenesis enhanced by IH.Methods The proliferation of neural stem cells (NSCs) was identified by Brdu labeling.The differentiation and migration of NSCs were identified by Brdu and NeuN double labeling.The morphology of astrocyte was identified by GFAP immunohistochemistry staining.Thyl-transgenic mice were used to identified the structure of spine.Notch l deficiency mice were used to identified the role of Notchl.Results (1) Intermittent hypoxia could promote the neurogenesis in DG of hippocampus, including proliferation of NSCs, migration of neuron, development of spine and astrocyte.(2) The promoted effects of intermittent hypoxia on neurogenesis were inhibited in Notchl deficiency mice.Conclusion Intermittent hypoxia could promote the neurognesis in DG, which was mediated through Notchl signaling pathway.