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Multiple sclerosis (MS) is considered to be an autoimmune-mediated demyelinating disease of central nervous system (CNS), with a hallmark of extensive demyelination in the CNS.Effective remyelination is critical in relieving diseases.Electroacupuncture (EA), originated from traditional Chinese medicine, has been widely used to treat CNS diseases all over the world.Several studies display that EA could ameliorate the symptoms of multiple sclerosis clinically and promote remyelination on spinal cord injury model of rat, but the role of EA in demyelinating diseases are barely known.In this study, we examined the remyelinating properties and mechanisms of EA in cuprizone(CPZ)-induced demyelinating model, a well-characterized model specifically to unravel the demyelination and remyelination mechanism in MS.The CPZ-induced model was induced by feeding male C57BL/6 mice aged between 5 and 6 weeks with standard chow containing 0.2% CPZ for 5 weeks.After 5 weeks induction, CPZ was removed and replaced with standard diet for 2 weeks allowing for spontaneous remyelination.EA treatment started from the first day of week 5 after the mice receiving CPZ chow and lasted for 3 weeks.Two acupoints in the govemor vessel (GV), Baihui (GV20) and Zhiyang (GV9) were adopted.Mice received 30min EA treatment once every other day.The following results were obtained: 1) EA treatment significantly attenuates neurobehavioral dysfunctions of demyelinating mice.2) EA promotes remyelination in corpus callosum.3) EA enhances the clearance and removal of degraded myelin debris in corpus callosum.4) More microglial cells displaying ameboid, phagocytic shape with M2 phenotype are infiltrated into corpus callosum after 3 weeks successive EA treatment.We conclude that EA can promote remyelination by recruiting more phagocytic microglial cells into corpus callosum removing degraded myelin debris and ameliorate neurobehaviors.Therefore, EA may have a potential therapeutic value as an altemative treatment for demyelinating diseases including multiple sclerosis.