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Objective: The molecular weight and purity of Dendrobium officinale polysaccharides were determined by gel permeation chromatography(GPC).Method: The Waters Ultrahydrogel TM linear column(7.8 × 300 mm)and 2414(RID)refractive index detector were used,the mobile phase was ultrapure water(Millipore,Milli-Q Advantage A10),flow rate was 1.0 min/mL,column temperature was 55 ℃ and injection volume was 30 μL.