Protein phosphorylation catalyzed by protein kinases is one of the most important posttranslational modification mechanisms in the process of cellular signal transduction,which plays a critical regulatory role in many fundamental biological processes.Aberration in protein kinase activities,leading to abnormal protein phosphorylation states,can results in a number of human diseases.Protein kinase are a group of especially important targets for drug therapy.Therefore,sensitive detection of protein kinase activity are of great significance for further understanding the cellular signal transduction,clinical diagnosis,and development of targeted therapy.